Sittingbourne FC visit Crabble tonight as Dover start their defence of the Kent Reliance Senior Cup.
Whites lifted the trophy in May, beating Welling United 2-0 in the final in Maidstone.
Boss Chris Kinnear is expected to name a much changed side for the Round Two clash, with those that started Saturday’s home National League win over Chester likely to be rested.
Opponents Sittingbourne beat Faversham 2-1 in the last round, thanks to a stoppage-time winner from sub Keanu Williams.
Sam Berwick had headed the home side in front before the break, with Jono Richardson levelling for the Brickies shortly after the hour mark.
Sittingbourne are currently fifth in the Bostik South standings.
The kicks-off at 7.45pm and the club have reduced the admission price for the game down to just £5 for adults, while under-11s get in for free.