The Dover Academy conceded a 96th minute penalty to deny them of all three points at Eastleigh.
Noah Carney added to his goal last week in the FA Youth Cup by scoring twice as the young Whites looked set to take victory.
However, Eastleigh were gifted an equaliser deep into added time, when a lapse in concentration allowed the hosts to score late on.
Dover started strongly and took the lead midway through the first half through some excellent play from Rolando Onu, who in turn set up Carney to finish.
The young whites almost doubled their lead towards the break, however George Lamb’s effort struck the post.
Carney doubled his and Whites’ tally in the second half after some good play allowed the youngster to coolly stroke home.
Eastleigh were then awarded a spot kick, however Athletic goalkeeper Magnus Lewis guessed correctly and saved the penalty.
However, the Spitfires pulled level late on, halving the deficit with 10 minutes remaining before being awarded a second spot-kick, this time after 90 minutes had been played, which was duly converted to level the scores.
About the game, Academy manager Mike Sandmann said: “I can’t fault the lads for their hard work but we need to be better in difficult moments in games and learn how to manage games better.
“It’s a learning curve for the lads with many still only in their first year, so it’s something we will be looking to improve on as we continue through the season.”